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Identification of an inhibitor-binding site to HIV-1 integrase with affinity acetylation and mass spectrometry
We report a methodology that combines affinity acetylation with MS analysis for accurate mapping of an inhibitor-binding site to a target protein. For this purpose, we used a known HIV-1 integrase inhibitor containing aryl di-O-acetyl groups (Acetylated-Inhibitor). In addition, we designed a control...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2004
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC406438/ https://ncbi.nlm.nih.gov/pubmed/15118107 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0400873101 |
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