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Identification of specific HIV-1 reverse transcriptase contacts to the viral RNA:tRNA complex by mass spectrometry and a primary amine selective reagent

We have devised a high-resolution protein footprinting methodology to dissect HIV-1 reverse transcriptase (RT) contacts to the viral RNA:tRNA complex. The experimental strategy included modification of surface-exposed lysines in RT and RT-viral RNA:tRNA complexes by the primary amine selective reage...

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Detalhes bibliográficos
Main Authors: Kvaratskhelia, Mamuka, Miller, Jennifer T., Budihas, Scott R., Pannell, Lewis K., Le Grice, Stuart F. J.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2002
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC138552/
https://ncbi.nlm.nih.gov/pubmed/12461175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.252550199
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