Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

BACKGROUND: Recent successes in the determination of G-protein coupled receptor (GPCR) structures have relied on the ability of receptor variants to overcome difficulties in expression and purification. Therefore, the quick screening of functionally expressed stable receptor variants is vital. RESUL...

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Detalhes bibliográficos
Main Authors: Shiroishi, Mitsunori, Tsujimoto, Hirokazu, Makyio, Hisayoshi, Asada, Hidetsugu, Yurugi-Kobayashi, Takami, Shimamura, Tatsuro, Murata, Takeshi, Nomura, Norimichi, Haga, Tatsuya, Iwata, So, Kobayashi, Takuya
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2012
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3495400/
https://ncbi.nlm.nih.gov/pubmed/22694812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-11-78
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