Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

BACKGROUND: Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled receptors (GPCRs) for structural studies. Rec...

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Main Authors: Asada, Hidetsugu, Uemura, Tomoko, Yurugi-Kobayashi, Takami, Shiroishi, Mitsunori, Shimamura, Tatsuro, Tsujimoto, Hirokazu, Ito, Keisuke, Sugawara, Taishi, Nakane, Takanori, Nomura, Norimichi, Murata, Takeshi, Haga, Tatsuya, Iwata, So, Kobayashi, Takuya
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2011
Assuntos:
Research
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3094209/
https://ncbi.nlm.nih.gov/pubmed/21513509
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-24
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