Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

BACKGROUND: Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled receptors (GPCRs) for structural studies. Rec...

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Main Authors: Asada, Hidetsugu, Uemura, Tomoko, Yurugi-Kobayashi, Takami, Shiroishi, Mitsunori, Shimamura, Tatsuro, Tsujimoto, Hirokazu, Ito, Keisuke, Sugawara, Taishi, Nakane, Takanori, Nomura, Norimichi, Murata, Takeshi, Haga, Tatsuya, Iwata, So, Kobayashi, Takuya
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2011
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3094209/
https://ncbi.nlm.nih.gov/pubmed/21513509
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-24
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