Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis

BACKGROUND: Various protein expression systems, such as Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae), Pichia pastoris (P. pastoris), insect cells and mammalian cell lines, have been developed for the synthesis of G protein-coupled receptors (GPCRs) for structural studies. Rec...

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Bibliografiska uppgifter
Huvudupphovsmän: Asada, Hidetsugu, Uemura, Tomoko, Yurugi-Kobayashi, Takami, Shiroishi, Mitsunori, Shimamura, Tatsuro, Tsujimoto, Hirokazu, Ito, Keisuke, Sugawara, Taishi, Nakane, Takanori, Nomura, Norimichi, Murata, Takeshi, Haga, Tatsuya, Iwata, So, Kobayashi, Takuya
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2011
Ämnen:
Research
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3094209/
https://ncbi.nlm.nih.gov/pubmed/21513509
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-10-24
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