Platform for the rapid construction and evaluation of GPCRs for crystallography in Saccharomyces cerevisiae

BACKGROUND: Recent successes in the determination of G-protein coupled receptor (GPCR) structures have relied on the ability of receptor variants to overcome difficulties in expression and purification. Therefore, the quick screening of functionally expressed stable receptor variants is vital. RESUL...

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Main Authors: Shiroishi, Mitsunori, Tsujimoto, Hirokazu, Makyio, Hisayoshi, Asada, Hidetsugu, Yurugi-Kobayashi, Takami, Shimamura, Tatsuro, Murata, Takeshi, Nomura, Norimichi, Haga, Tatsuya, Iwata, So, Kobayashi, Takuya
格式: Artigo
語言:Inglês
出版: BioMed Central 2012
主題:
Research
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3495400/
https://ncbi.nlm.nih.gov/pubmed/22694812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-11-78
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