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Evidence for pH-Dependent Protease Activity in the Adeno-Associated Virus Capsid
Incubation of highly purified adeno-associated virus (AAV) capsids in vitro at pH 5.5 induced significant autocleavage of capsid proteins at several amino acid positions. No autocleavage was seen at pH 7.5. Examination of other AAV serotypes showed at least two different pH-induced cleavage patterns...
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| Główni autorzy: | , , , , , , , |
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| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American Society for Microbiology
2012
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3486322/ https://ncbi.nlm.nih.gov/pubmed/22915820 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.01717-12 |
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