Evidence for pH-Dependent Protease Activity in the Adeno-Associated Virus Capsid

Incubation of highly purified adeno-associated virus (AAV) capsids in vitro at pH 5.5 induced significant autocleavage of capsid proteins at several amino acid positions. No autocleavage was seen at pH 7.5. Examination of other AAV serotypes showed at least two different pH-induced cleavage patterns...

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Bibliografske podrobnosti
Main Authors: Salganik, Maxim, Venkatakrishnan, Balasubramanian, Bennett, Antonette, Lins, Bridget, Yarbrough, Joseph, Muzyczka, Nicholas, Agbandje-McKenna, Mavis, McKenna, Robert
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 2012
Teme:
Structure and Assembly
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3486322/
https://ncbi.nlm.nih.gov/pubmed/22915820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.01717-12
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