Evidence for pH-Dependent Protease Activity in the Adeno-Associated Virus Capsid

Incubation of highly purified adeno-associated virus (AAV) capsids in vitro at pH 5.5 induced significant autocleavage of capsid proteins at several amino acid positions. No autocleavage was seen at pH 7.5. Examination of other AAV serotypes showed at least two different pH-induced cleavage patterns...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Salganik, Maxim, Venkatakrishnan, Balasubramanian, Bennett, Antonette, Lins, Bridget, Yarbrough, Joseph, Muzyczka, Nicholas, Agbandje-McKenna, Mavis, McKenna, Robert
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: American Society for Microbiology 2012
Pynciau:
Structure and Assembly
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC3486322/
https://ncbi.nlm.nih.gov/pubmed/22915820
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JVI.01717-12
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