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A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells
We have developed a new technique for proximity-dependent labeling of proteins in eukaryotic cells. Named BioID for proximity-dependent biotin identification, this approach is based on fusion of a promiscuous Escherichia coli biotin protein ligase to a targeting protein. BioID features proximity-dep...
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| Hlavní autoři: | , , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
The Rockefeller University Press
2012
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3308701/ https://ncbi.nlm.nih.gov/pubmed/22412018 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201112098 |
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