An improved smaller biotin ligase for BioID proximity labeling

The BioID method uses a promiscuous biotin ligase to detect protein–protein associations as well as proximate proteins in living cells. Here we report improvements to the BioID method centered on BioID2, a substantially smaller promiscuous biotin ligase. BioID2 enables more-selective targeting of fu...

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Udgivet i:Mol Biol Cell
Main Authors: Kim, Dae In, Jensen, Samuel C., Noble, Kyle A., KC, Birendra, Roux, Kenneth H., Motamedchaboki, Khatereh, Roux, Kyle J.
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Cell Biology 2016
Fag:
Brief Reports
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4831873/
https://ncbi.nlm.nih.gov/pubmed/26912792
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1091/mbc.E15-12-0844
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