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BioID: A Screen for Protein-Protein Interactions

BioID is a unique method to screen for physiologically relevant protein interactions that occur in living cells. This technique harnesses a promiscuous biotin ligase to biotinylate proteins based on proximity. The ligase is fused to a protein of interest and expressed in cells, where it biotinylates...

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Detalhes bibliográficos
Publicado no:Curr Protoc Protein Sci
Main Authors: Roux, Kyle J., Kim, Dae In, Burke, Brian, May, Danielle G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6028010/
https://ncbi.nlm.nih.gov/pubmed/29516480
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cpps.51
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