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Promiscuous protein biotinylation by Escherichia coli biotin protein ligase
Biotin protein ligases (BPLs) are enzymes of extraordinary specificity. BirA, the BPL of Escherichia coli biotinylates only a single cellular protein. We report a mutant BirA that attaches biotin to a large number of cellular proteins in vivo and to bovine serum albumin, chloramphenicol acetyltransf...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Cold Spring Harbor Laboratory Press
2004
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2286582/ https://ncbi.nlm.nih.gov/pubmed/15459338 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04911804 |
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