Expression, biotinylation and purification of a biotin-domain peptide from the biotin carboxy carrier protein of Escherichia coli acetyl-CoA carboxylase.

A protein segment consisting of the C-terminal 87 residues of the biotin carboxy carrier protein from Escherichia coli acetyl-CoA carboxylase was overexpressed in E. coli. The expressed biotin-domain peptide can be fully biotinylated by coexpression with a plasmid that overproduces E. coli biotin li...

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Main Authors: Chapman-Smith, A, Turner, D L, Cronan, J E, Morris, T W, Wallace, J C
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1137313/
https://ncbi.nlm.nih.gov/pubmed/7945216
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