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Promiscuous protein biotinylation by Escherichia coli biotin protein ligase

Biotin protein ligases (BPLs) are enzymes of extraordinary specificity. BirA, the BPL of Escherichia coli biotinylates only a single cellular protein. We report a mutant BirA that attaches biotin to a large number of cellular proteins in vivo and to bovine serum albumin, chloramphenicol acetyltransf...

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Bibliografske podrobnosti
Main Authors: Choi-Rhee, Eunjoo, Schulman, Howard, Cronan, John E.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2004
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2286582/
https://ncbi.nlm.nih.gov/pubmed/15459338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.04911804
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