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Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin
This protocol describes a simple and efficient way to label specific cell surface proteins with biophysical probes on mammalian cells. Cell surface proteins tagged with a 15-amino acid peptide are biotinylated by Escherichia coli biotin ligase (BirA), whereas endogenous proteins are not modified. Th...
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| Main Authors: | , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2008
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2671200/ https://ncbi.nlm.nih.gov/pubmed/18323822 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.20 |
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