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Imaging proteins in live mammalian cells with biotin ligase and monovalent streptavidin

This protocol describes a simple and efficient way to label specific cell surface proteins with biophysical probes on mammalian cells. Cell surface proteins tagged with a 15-amino acid peptide are biotinylated by Escherichia coli biotin ligase (BirA), whereas endogenous proteins are not modified. Th...

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Detalles Bibliográficos
Autores principales: Howarth, Mark, Ting, Alice Y
Formato: Artigo
Lenguaje:Inglês
Publicado: 2008
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2671200/
https://ncbi.nlm.nih.gov/pubmed/18323822
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2008.20
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