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Quantum Dot Targeting with Lipoic Acid Ligase and HaloTag for Single Molecule Imaging on Living Cells

We present a methodology for targeting quantum dots to proteins on living cells in two steps. In the first step, E. coli lipoic acid ligase (LplA) site-specifically attaches 10-bromodecanoic acid onto a 13-amino acid peptide that can be genetically fused to a protein of interest. In the second step,...

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Bibliografiske detaljer
Main Authors: Liu, Daniel S., Phipps, William S., Loh, Ken H., Howarth, Mark, Ting, Alice Y.
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3528850/
https://ncbi.nlm.nih.gov/pubmed/23181687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/nn304793z
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