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Targeting quantum dots to surface proteins in living cells with biotin ligase
Escherichia coli biotin ligase site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (AP) sequence. We show that mammalian cell surface proteins tagged with AP can be biotinylated by biotin ligase added to the medium, while endogenous proteins remain unmodified....
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1129026/ https://ncbi.nlm.nih.gov/pubmed/15897449 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0503125102 |
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