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A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells
We have developed a new technique for proximity-dependent labeling of proteins in eukaryotic cells. Named BioID for proximity-dependent biotin identification, this approach is based on fusion of a promiscuous Escherichia coli biotin protein ligase to a targeting protein. BioID features proximity-dep...
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Asıl Yazarlar: | , , , |
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Materyal Türü: | Artigo |
Dil: | Inglês |
Baskı/Yayın Bilgisi: |
The Rockefeller University Press
2012
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Konular: | |
Online Erişim: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3308701/ https://ncbi.nlm.nih.gov/pubmed/22412018 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201112098 |
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