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A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

We have developed a new technique for proximity-dependent labeling of proteins in eukaryotic cells. Named BioID for proximity-dependent biotin identification, this approach is based on fusion of a promiscuous Escherichia coli biotin protein ligase to a targeting protein. BioID features proximity-dep...

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Detalhes bibliográficos
Main Authors: Roux, Kyle J., Kim, Dae In, Raida, Manfred, Burke, Brian
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3308701/
https://ncbi.nlm.nih.gov/pubmed/22412018
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1083/jcb.201112098
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