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Real-Time PCR Assay for Detection and Enumeration of Dekkera bruxellensis in Wine

Traditional methods to detect the spoilage yeast Dekkera bruxellensis from wine involve lengthy enrichments. To overcome this difficulty, we developed a quantitative real-time PCR method to directly detect and enumerate D. bruxellensis in wine. Specific PCR primers to D. bruxellensis were designed t...

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Bibliographische Detailangaben
Hauptverfasser: Phister, Trevor G., Mills, David A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2003
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC310000/
https://ncbi.nlm.nih.gov/pubmed/14660395
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.69.12.7430-7434.2003
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