Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein

The RadA intein from the hyperthermophilic archaebacterium Pyrococcus horikoshii was cloned, expressed and purified for subsequent structure determination. The protein crystallized rapidly in several conditions. The best crystals, which diffracted to 1.75 Å resolution, were harvested from drops cons...

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Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Lyskowski, Andrzej, Oeemig, Jesper S., Jaakkonen, Anniina, Rommi, Katariina, DiMaio, Frank, Zhou, Dongwen, Kajander, Tommi, Baker, David, Wlodawer, Alexander, Goldman, Adrian, Iwaï, Hideo
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: International Union of Crystallography 2011
Gaiak:
Crystallization Communications
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC3087655/
https://ncbi.nlm.nih.gov/pubmed/21543876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111008372
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