Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein

The RadA intein from the hyperthermophilic archaebacterium Pyrococcus horikoshii was cloned, expressed and purified for subsequent structure determination. The protein crystallized rapidly in several conditions. The best crystals, which diffracted to 1.75 Å resolution, were harvested from drops cons...

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Hlavní autoři: Lyskowski, Andrzej, Oeemig, Jesper S., Jaakkonen, Anniina, Rommi, Katariina, DiMaio, Frank, Zhou, Dongwen, Kajander, Tommi, Baker, David, Wlodawer, Alexander, Goldman, Adrian, Iwaï, Hideo
Médium: Artigo
Jazyk:Inglês
Vydáno: International Union of Crystallography 2011
Témata:
Crystallization Communications
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3087655/
https://ncbi.nlm.nih.gov/pubmed/21543876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111008372
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