Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein

The RadA intein from the hyperthermophilic archaebacterium Pyrococcus horikoshii was cloned, expressed and purified for subsequent structure determination. The protein crystallized rapidly in several conditions. The best crystals, which diffracted to 1.75 Å resolution, were harvested from drops cons...

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Main Authors: Lyskowski, Andrzej, Oeemig, Jesper S., Jaakkonen, Anniina, Rommi, Katariina, DiMaio, Frank, Zhou, Dongwen, Kajander, Tommi, Baker, David, Wlodawer, Alexander, Goldman, Adrian, Iwaï, Hideo
Formáid: Artigo
Teanga:Inglês
Foilsithe: International Union of Crystallography 2011
Ábhair:
Crystallization Communications
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3087655/
https://ncbi.nlm.nih.gov/pubmed/21543876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111008372
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