Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein

The RadA intein from the hyperthermophilic archaebacterium Pyrococcus horikoshii was cloned, expressed and purified for subsequent structure determination. The protein crystallized rapidly in several conditions. The best crystals, which diffracted to 1.75 Å resolution, were harvested from drops cons...

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Bibliografische gegevens
Hoofdauteurs: Lyskowski, Andrzej, Oeemig, Jesper S., Jaakkonen, Anniina, Rommi, Katariina, DiMaio, Frank, Zhou, Dongwen, Kajander, Tommi, Baker, David, Wlodawer, Alexander, Goldman, Adrian, Iwaï, Hideo
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: International Union of Crystallography 2011
Onderwerpen:
Crystallization Communications
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3087655/
https://ncbi.nlm.nih.gov/pubmed/21543876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111008372
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