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Efficient generation of hydrazides in proteins by RadA split intein
Protein C-terminal hydrazides are useful for bioconjugation and construction of proteins from multiple fragments via native chemical ligation. To generate C-terminal hydrazides in proteins, we developed an efficient intein-based preparation method using thiols and hydrazine to accelerate the formati...
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| Publicado en: | Chembiochem |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
2019
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7027934/ https://ncbi.nlm.nih.gov/pubmed/31265209 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201900160 |
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