Cloning, expression, purification, crystallization and preliminary X-ray diffraction data of the Pyrococcus horikoshii RadA intein

The RadA intein from the hyperthermophilic archaebacterium Pyrococcus horikoshii was cloned, expressed and purified for subsequent structure determination. The protein crystallized rapidly in several conditions. The best crystals, which diffracted to 1.75 Å resolution, were harvested from drops cons...

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Main Authors: Lyskowski, Andrzej, Oeemig, Jesper S., Jaakkonen, Anniina, Rommi, Katariina, DiMaio, Frank, Zhou, Dongwen, Kajander, Tommi, Baker, David, Wlodawer, Alexander, Goldman, Adrian, Iwaï, Hideo
Formato: Artigo
Idioma:Inglês
Publicado: International Union of Crystallography 2011
Assuntos:
Crystallization Communications
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3087655/
https://ncbi.nlm.nih.gov/pubmed/21543876
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309111008372
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