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Kinetic isotope effects for RNA cleavage by 2'-O- transphosphorylation: Nucleophilic activation by specific base

To better understand the interactions between catalysts and transition states during RNA strand cleavage, primary (18)O kinetic isotope effects and solvent D(2)O isotope effects were measured to probe the mechanism of base-catalyzed 2'-O-transphosphorylation of the RNA dinucleotide 5'-UpG-...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Harris, Michael E, Dai, Qing, Gu, Hong, Kellerman, Dan, Piccirilli, Joseph A, Anderson, Vernon E
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2946848/
https://ncbi.nlm.nih.gov/pubmed/20669950
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja103550e
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