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Kinetic isotope effects for RNA cleavage by 2'-O- transphosphorylation: Nucleophilic activation by specific base

To better understand the interactions between catalysts and transition states during RNA strand cleavage, primary (18)O kinetic isotope effects and solvent D(2)O isotope effects were measured to probe the mechanism of base-catalyzed 2'-O-transphosphorylation of the RNA dinucleotide 5'-UpG-...

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Detalhes bibliográficos
Main Authors: Harris, Michael E, Dai, Qing, Gu, Hong, Kellerman, Dan, Piccirilli, Joseph A, Anderson, Vernon E
Formato: Artigo
Idioma:Inglês
Publicado em: 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2946848/
https://ncbi.nlm.nih.gov/pubmed/20669950
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja103550e
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