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A Fluopol-ABPP HTS Assay to Identify PAD Inhibitors

Protein Arginine Deiminase (PAD) activity is dysregulated in numerous diseases, e.g., Rheumatoid Arthritis. Herein we describe the development of a fluorescence polarization-Activity Based Protein Profiling (fluopol-ABPP) based high throughput screening assay that can be used to identify PAD-selecti...

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Main Authors: Knuckley, Bryan, Jones, Justin E., Bachovchin, Daniel A., Slack, Jessica, Causey, Corey P., Brown, Steven J., Rosen, Hugh, Cravatt, Benjamin F., Thompson, Paul R.
Formato: Artigo
Idioma:Inglês
Publicado: 2010
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2943038/
https://ncbi.nlm.nih.gov/pubmed/20740228
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c0cc02634d
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