Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology

We previously demonstrated high-frequency, targeted DNA addition mediated by the homology-directed DNA repair pathway. This method uses a zinc-finger nuclease (ZFN) to create a site-specific double-strand break (DSB) that facilitates copying of genetic information into the chromosome from an exogeno...

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Autori principali: Orlando, Salvatore J., Santiago, Yolanda, DeKelver, Russell C., Freyvert, Yevgeniy, Boydston, Elizabeth A., Moehle, Erica A., Choi, Vivian M., Gopalan, Sunita M., Lou, Jacqueline F., Li, James, Miller, Jeffrey C., Holmes, Michael C., Gregory, Philip D., Urnov, Fyodor D., Cost, Gregory J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2010
Soggetti:
Methods Online
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2926620/
https://ncbi.nlm.nih.gov/pubmed/20530528
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkq512
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