Functional genomics, proteomics, and regulatory DNA analysis in isogenic settings using zinc finger nuclease-driven transgenesis into a safe harbor locus in the human genome

Isogenic settings are routine in model organisms, yet remain elusive for genetic experiments on human cells. We describe the use of designed zinc finger nucleases (ZFNs) for efficient transgenesis without drug selection into the PPP1R12C gene, a “safe harbor” locus known as AAVS1. ZFNs enable target...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: DeKelver, Russell C., Choi, Vivian M., Moehle, Erica A., Paschon, David E., Hockemeyer, Dirk, Meijsing, Sebastiaan H., Sancak, Yasemin, Cui, Xiaoxia, Steine, Eveline J., Miller, Jeffrey C., Tam, Phillip, Bartsevich, Victor V., Meng, Xiangdong, Rupniewski, Igor, Gopalan, Sunita M., Sun, Helena C., Pitz, Kathleen J., Rock, Jeremy M., Zhang, Lei, Davis, Gregory D., Rebar, Edward J., Cheeseman, Iain M., Yamamoto, Keith R., Sabatini, David M., Jaenisch, Rudolf, Gregory, Philip D., Urnov, Fyodor D.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2010
Materias:
Method
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2909576/
https://ncbi.nlm.nih.gov/pubmed/20508142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.106773.110
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares