Llwytho...

High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis

We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed the effects of all possible single-nucleotide mutations for three b...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Patwardhan, Rupali P, Lee, Choli, Litvin, Oren, Young, David L, Pe'er, Dana, Shendure, Jay
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2009
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC2849652/
https://ncbi.nlm.nih.gov/pubmed/19915551
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.1589
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