Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci

Cas9-mediated, high-throughput, saturating in situ mutagenesis permits fine-mapping of function across genomic segments. Disease- and trait-associated variants from genome-wide association studies largely cluster in regulatory DNA. Here we demonstrate the use of multiple designer nucleases and varia...

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Publicado no:Nat Genet
Main Authors: Canver, Matthew C., Lessard, Samuel, Pinello, Luca, Wu, Yuxuan, Ilboudo, Yann, Stern, Emily N., Needleman, Austen J., Galactéros, Frédéric, Brugnara, Carlo, Kutlar, Abdullah, McKenzie, Colin, Reid, Marvin, Chen, Diane D., Das, Partha Pratim, Cole, Mitchel, Zeng, Jing, Kurita, Ryo, Nakamura, Yukio, Yuan, Guo-Cheng, Lettre, Guillaume, Bauer, Daniel E., Orkin, Stuart H.
Formato: Artigo
Idioma:Inglês
Publicado em: 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5374001/
https://ncbi.nlm.nih.gov/pubmed/28218758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ng.3793
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