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High-resolution analysis of DNA regulatory elements by synthetic saturation mutagenesis
We present a method that harnesses massively parallel DNA synthesis and sequencing for the high-throughput functional analysis of regulatory sequences at single-nucleotide resolution. As a proof of concept, we quantitatively assayed the effects of all possible single-nucleotide mutations for three b...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2849652/ https://ncbi.nlm.nih.gov/pubmed/19915551 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.1589 |
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