Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography

We present a simple and fast method for large-scale purification of RNA oligonucleotides suitable for biochemical and structural studies. RNAs are transcribed in vitro with T7 RNA polymerase using linearized plasmid DNA templates. After addition of EDTA, the crude transcription reaction is subjected...

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Hlavní autoři: Easton, Laura E., Shibata, Yoko, Lukavsky, Peter J.
Médium: Artigo
Jazyk:Inglês
Vydáno: Cold Spring Harbor Laboratory Press 2010
Témata:
Method
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2822929/
https://ncbi.nlm.nih.gov/pubmed/20100812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1862210
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