Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography

We present a simple and fast method for large-scale purification of RNA oligonucleotides suitable for biochemical and structural studies. RNAs are transcribed in vitro with T7 RNA polymerase using linearized plasmid DNA templates. After addition of EDTA, the crude transcription reaction is subjected...

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Bibliografski detalji
Glavni autori: Easton, Laura E., Shibata, Yoko, Lukavsky, Peter J.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2010
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Method
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2822929/
https://ncbi.nlm.nih.gov/pubmed/20100812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1862210
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