Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography

We present a simple and fast method for large-scale purification of RNA oligonucleotides suitable for biochemical and structural studies. RNAs are transcribed in vitro with T7 RNA polymerase using linearized plasmid DNA templates. After addition of EDTA, the crude transcription reaction is subjected...

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Detalhes bibliográficos
Main Authors: Easton, Laura E., Shibata, Yoko, Lukavsky, Peter J.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2010
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2822929/
https://ncbi.nlm.nih.gov/pubmed/20100812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1862210
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