Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography

We present a simple and fast method for large-scale purification of RNA oligonucleotides suitable for biochemical and structural studies. RNAs are transcribed in vitro with T7 RNA polymerase using linearized plasmid DNA templates. After addition of EDTA, the crude transcription reaction is subjected...

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Bibliografiske detaljer
Main Authors: Easton, Laura E., Shibata, Yoko, Lukavsky, Peter J.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2010
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Method
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2822929/
https://ncbi.nlm.nih.gov/pubmed/20100812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1862210
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