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A general method for rapid and nondenaturing purification of RNAs

A key bottleneck in RNA structural studies is preparing milligram quantities of RNA, and current techniques have changed little in over a decade. To address this, we have developed an affinity tag-based purification method of RNA oligonucleotides. The tag is attached to the 3′-end of almost any desi...

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Autors principals: KIEFT, JEFFREY S., BATEY, ROBERT T.
Format: Artigo
Idioma:Inglês
Publicat: Copyright 2004 by RNA Society 2004
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370590/
https://ncbi.nlm.nih.gov/pubmed/15146082
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.7040604
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