Rapid purification of RNAs using fast performance liquid chromatography (FPLC)

We present here an improved RNA purification method using fast performance liquid chromatography (FPLC) size-exclusion chromatography in place of denaturing polyacrylamide gel electrophoresis (PAGE). The method allows preparation of milligram quantities of pure RNA in a single day. As RNA oligonucle...

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Bibliografiske detaljer
Main Authors: Kim, Insil, McKenna, Sean A., Viani Puglisi, Elisabetta, Puglisi, Joseph D.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2007
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Method
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1781364/
https://ncbi.nlm.nih.gov/pubmed/17179067
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.342607
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