Strong anion-exchange fast performance liquid chromatography as a versatile tool for preparation and purification of RNA produced by in vitro transcription

Here we demonstrate the use of strong anion-exchange fast performance liquid chromatography (FPLC) as a simple, fast, and robust method for RNA production by in vitro transcription. With this technique, we have purified different transcription templates from unreacted reagents in large quantities. T...

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Detalhes bibliográficos
Main Authors: Koubek, Jiri, Lin, Ku Feng, Chen, Yet Ran, Cheng, Richard Ping, Huang, Joseph Jen Tse
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2013
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3854534/
https://ncbi.nlm.nih.gov/pubmed/23929938
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.038117.113
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