Rapid, nondenaturing RNA purification using weak anion-exchange fast performance liquid chromatography

We present a simple and fast method for large-scale purification of RNA oligonucleotides suitable for biochemical and structural studies. RNAs are transcribed in vitro with T7 RNA polymerase using linearized plasmid DNA templates. After addition of EDTA, the crude transcription reaction is subjected...

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Autores principales: Easton, Laura E., Shibata, Yoko, Lukavsky, Peter J.
Formato: Artigo
Lenguaje:Inglês
Publicado: Cold Spring Harbor Laboratory Press 2010
Materias:
Method
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2822929/
https://ncbi.nlm.nih.gov/pubmed/20100812
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1862210
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