Buffer Optimization of Thermal Melt Assays of Plasmodium Proteins for Detection of Small-Molecule Ligands

In the last decade, thermal melt/thermal shift assays have become a common tool for identifying ligands and other factors that stabilize specific proteins. Increased stability is indicated by an increase in the protein's melting temperature (T(m)). In optimizing the assays for subsequent screen...

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Detaylı Bibliyografya
Asıl Yazarlar: Crowther, Gregory J., Napuli, Alberto J., Thomas, Andrew P., Chung, Diana J., Kovzun, Kuzma V., Leibly, David J., Castaneda, Lisa J., Bhandari, Janhavi, Damman, Christopher J., Hui, Raymond, Hol, Wim G. J., Buckner, Frederick S., Verlinde, Christophe L. M. J., Zhang, Zhongsheng, Fan, Erkang, Van Voorhis, Wesley C.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2009
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2819745/
https://ncbi.nlm.nih.gov/pubmed/19470714
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/1087057109335749
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