Use of thermal melt curves to assess the quality of enzyme preparations()

This study sought to determine whether the quality of enzyme preparations can be determined from their melting curves, which may easily be obtained using a fluorescent probe and a standard RT-PCR machine. Thermal melt data on 31 recombinant enzymes from Plasmodium parasites were acquired by incremen...

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Detalhes bibliográficos
Main Authors: Crowther, Gregory J., He, Panqing, Rodenbough, Philip P., Thomas, Andrew P., Kovzun, Kuzma V., Leibly, David J., Bhandari, Janhavi, Castaneda, Lisa J., Hol, Wim G. J., Gelb, Michael H., Napuli, Alberto J., Van Voorhis, Wesley C.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3270315/
https://ncbi.nlm.nih.gov/pubmed/20018159
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.12.018
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