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Use of thermal melt curves to assess the quality of enzyme preparations()

This study sought to determine whether the quality of enzyme preparations can be determined from their melting curves, which may easily be obtained using a fluorescent probe and a standard RT-PCR machine. Thermal melt data on 31 recombinant enzymes from Plasmodium parasites were acquired by incremen...

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Detaylı Bibliyografya
Asıl Yazarlar: Crowther, Gregory J., He, Panqing, Rodenbough, Philip P., Thomas, Andrew P., Kovzun, Kuzma V., Leibly, David J., Bhandari, Janhavi, Castaneda, Lisa J., Hol, Wim G. J., Gelb, Michael H., Napuli, Alberto J., Van Voorhis, Wesley C.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2009
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC3270315/
https://ncbi.nlm.nih.gov/pubmed/20018159
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.12.018
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