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Formation and Stability of a Vinyl Carbanion at the Active Site of Orotidine 5′-Monophosphate Decarboxylase: pK(a) of the C-6 Proton of Enzyme-Bound UMP

[Image: see text] We report that orotidine 5′-monophosphate decarboxylase (OMPDC) catalyzes exchange of the C-6 proton of uridine 5′-monophosphate (UMP) for deuterium from solvent in D(2)O at 25 °C and pD 7.0 – 9.3. Kinetic analysis of deuterium exchange gives pK(a) ≤ 22 for carbon deprotonation of...

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Xehetasun bibliografikoak
Egile Nagusiak: Amyes, Tina L., Wood, Bryant M., Chan, Kui, Gerlt, John A., Richard, John P.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 2008
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC2652670/
https://ncbi.nlm.nih.gov/pubmed/18186641
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja710384t
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