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Exploring the capacity of minimalist protein interfaces: interface energetics and affinity maturation to picomolar K(D) of a single-domain antibody with a flat paratope

A major architectural class in engineered binding proteins (“antibody mimics”) involves the presentation of recognition loops off a single-domain scaffold. This class of binding proteins, both natural and synthetic, has a strong tendency to bind a preformed cleft using a convex binding interface (pa...

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Autors principals: Koide, Akiko, Tereshko, Valentia, Uysal, Serdar, Margalef, Katrina, Kossiakoff, Anthony A., Koide, Shohei
Format: Artigo
Idioma:Inglês
Publicat: 2007
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2148503/
https://ncbi.nlm.nih.gov/pubmed/17888451
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2007.08.027
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