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Toward chaperone-assisted crystallography: Protein engineering enhancement of crystal packing and X-ray phasing capabilities of a camelid single-domain antibody (V(H)H) scaffold

A crystallization chaperone is an auxiliary protein that binds to a target of interest, enhances and modulates crystal packing, and provides high-quality phasing information. We critically evaluated the effectiveness of a camelid single-domain antibody (V(H)H) as a crystallization chaperone. By usin...

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Bibliografiska uppgifter
Huvudupphovsmän: Tereshko, Valentina, Uysal, Serdar, Koide, Akiko, Margalef, Katrina, Koide, Shohei, Kossiakoff, Anthony A.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Cold Spring Harbor Laboratory Press 2008
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2442005/
https://ncbi.nlm.nih.gov/pubmed/18445622
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1110/ps.034892.108
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