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Generating conformation-specific synthetic antibodies to trap proteins in selected functional states

A set of phage display sorting strategies and validation methodologies are presented that are capable of producing high performance synthetic antibodies (sABs) with customized properties. Exquisite control of antigen and conditions during the phage display selection process can yield sABs that: 1) r...

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Bibliographic Details
Main Authors: Paduch, Marcin, Koide, Akiko, Uysal, Serdar, Rizk, Shahir S., Koide, Shohei, Kossiakoff, Anthony A.
Format: Artigo
Language:Inglês
Published: 2012
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3650108/
https://ncbi.nlm.nih.gov/pubmed/23280336
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2012.12.010
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