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Optimization of a 12-Hour TaqMan PCR-Based Method for Detection of Salmonella Bacteria in Meat

We developed a 12-h Salmonella detection method, based on 8 h of preenrichment, followed by automated DNA extraction and a sensitive real-time PCR. The method was optimized to obtain the highest possible yield of cells and DNA. The growth of different Salmonella strains in various preenrichment medi...

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Hlavní autoři: Josefsen, M. H., Krause, M., Hansen, F., Hoorfar, J.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2007
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1892850/
https://ncbi.nlm.nih.gov/pubmed/17351094
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02823-06
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