Nanoliter scale PCR with TaqMan detection.

We monitored PCR in volumes of the order of 10 nl in glass microcapillaries using a fluorescence energy transfer assay in which fluorescence increases if product is made due to template-dependent nucleolytic degradation of an internally quenched probe (TaqMan assay). This assay detected single start...

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Main Authors: Kalinina, O, Lebedeva, I, Brown, J, Silver, J
Format: Artigo
Sprog:Inglês
Udgivet: 1997
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC146692/
https://ncbi.nlm.nih.gov/pubmed/9115368
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