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Nanoliter scale PCR with TaqMan detection.

We monitored PCR in volumes of the order of 10 nl in glass microcapillaries using a fluorescence energy transfer assay in which fluorescence increases if product is made due to template-dependent nucleolytic degradation of an internally quenched probe (TaqMan assay). This assay detected single start...

詳細記述

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書誌詳細
主要な著者: Kalinina, O, Lebedeva, I, Brown, J, Silver, J
フォーマット: Artigo
言語:Inglês
出版事項: 1997
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC146692/
https://ncbi.nlm.nih.gov/pubmed/9115368
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