Nanoliter scale PCR with TaqMan detection.
We monitored PCR in volumes of the order of 10 nl in glass microcapillaries using a fluorescence energy transfer assay in which fluorescence increases if product is made due to template-dependent nucleolytic degradation of an internally quenched probe (TaqMan assay). This assay detected single start...
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| Veröffentlicht in: | Nucleic Acids Res |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Oxford University Press
1997
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC146692/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9115368/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/25.10.1999 |
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