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Site-directed alkylation and the alternating access model for LacY
In a functional lactose permease mutant from Escherichia coli (LacY) devoid of native Cys residues, almost every residue was replaced individually with Cys and tested for reactivity with the permeant alkylating agent N-ethylmaleimide in right-side-out membrane vesicles. Here we present the results i...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
National Academy of Sciences
2007
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1766412/ https://ncbi.nlm.nih.gov/pubmed/17172438 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0609968104 |
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